Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain.

Data from ProteomeXchange, PXD ID: PXD000327. File: LG110324_BrH4_vs_Cere7_WT_DDA10_03.mzXML. Published as part of Proteomics. 2013 Nov 20 . From the Abstract: {{i}} ... In this study, two complementary approaches were used to assess the unique repertoire of proteins enriched within select regions of the adult mouse central nervous system (CNS), including the brain stem, cerebellum and remaining brain hemispheres. Of the 1200 proteins visualized by 2D-difference image gel electrophoresis (DIGE), approximately 150 (including cytosolic and membrane proteins) were found to exhibit statistically significant changes in relative abundance thus representing putative region-specific brain markers. In addition using a high precision 18 O-labeling strategy for the quantitative LC-MS/MS mapping of membrane proteins isolated from myelin-enriched fractions we have identified over 1000 proteins that have yet to be described in any other mammalian myelin proteome. ... {{/i}}