Transcriptomic analysis to identify the putative genes under the regulation of two component system BtrK/BtrR in C. acetobutylicum

To identify the putative genes under the regulation of two component system BtrK/BtrR, we performed a comparative transcriptomic analysis of the BtrK/BtrR overexpressing strain and the contron strain. Finally, we found that BtrK/BtrR was a global regulator and exerted pleiotropic regulatory role in C. acetobutylicum Samples for RNA extraction and microarray analysis were collected after 24 and 48 h of fermentation. From this analysis, 691 and 424 genes exhibited significantly altered transcription (fold change ≥ 2.0) after the overexpression of btrK/btrR at 24 and 48 h, respectively. Among these genes, 153 genes showed significantly different expression at both 24 and 48 h.