Differences in fetal longissimus muscle gene expression between low and high maternal nutrition

To investigate the impact of maternal nutrient restriction on skleletal muscle gene expression, we profiled gene expression in longissimus muscle of Japanese Black fetal calves in high and low nutrient condition of pregnant maternal cows . Japanese Black cows were fed with 60% (low-nutrition (LN) group, n=4) or 120% (high-nutrition (HN) group, n=4) of overall nutritional requirement during gestation. The fetal longissimus muscles were obtained at 8.5-mo post-conception of the maternal cows. The microarray-based gene expression was analyzed using the pooled total RNA samples for each of the nutritional treatment group.