Ago3 interaction proteins in BmN4 cell
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https://www.omicsdi.org/dataset/jpost/PXD023467
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We prepared BmN4 cell lysates in Co-IP buffer [30 mM HEPES-KOH pH 7.3, 150 mM potassium acetate, 5 mM magnesium acetate, 5 mM dithiothreitol, 0.1% (w/v) NP-40 and cOmplete ULTRA EDTA-free protease inhibitor (Roche)] with and without 1 mM EDTA and conducted immunoprecipitation assays using anti-Ago3 antibody. The reaction was terminated by boiling with SDS sample buffer.
创建时间：
2021-01-08
