FTO alleviates psoriasis-like dermatitis through the regulation of FN1

FTO was found to inhibit keratinocyte inflammation and proliferation in both in vitro and in vivo settings, independent of m6A demethylase function. RNA-seq was used to identify fibronectin (FN1,FN) as a potential target for FTO. FTO does not rely on demethylase activity to decrease FN1 expression. The anti-inflammatory and anti-proliferative effects of FTO on psoriasis partly depend on FN1. FTO may regulate the skipping of EDA exon in FN1. RNA sequencing was conducted on HaCaT cells that were either overexpressing an empty vector, wild-type FTO, or a mutant type that disrupts the demethylase activity.