Regulation of differentiation gene profile by PPARγ agonists in neural stem cells.

NSCs were cultured in NBM with EGF+bFGF in the presence of DMSO, ciglitazone, 15d-PGJ2 or ATRA for 3 days. The differentiation gene expression profile was analyzed by qRT-PCR using a 384 gene card array. The fold change was calculated using automatic threshold setting and is based on expression levels in DMSO treated cells as 1 after normalizing to 18S or GAPDH. The genes are arranged in the table from high to low expression in ciglitazone treated cells. ND, not detected and D, detected. Arrows indicate up or down regulated genes. This data is a representative of two independent experiments.