Spore viability of the different <i>rec114</i> alleles in various genetic backgrounds.

Spore viability was assessed following 2 day incubation on sporulation medium (SPM) plate at 30°C. Generally, 160 spores were scored for each strain except for those with (*) where 320 spores were analyzed. Viability was indicated as the fraction of viable spores over the total dissected. Abbreviations: T; threonine, S; serine, A; alanine, D; aspartic acid, E; glutamic acid, ND; not determined. 1Nature of mutations in rec114 alleles analyzed. 2Relevant genotypes of the strains to which REC114, rec114-8A, or the four different rec114-phosphomimetic alleles in the “REC114 allele” column were introduced to assess potential genetic interaction(s). 3Homozygous diploids expressing the indicated REC114 or rec114 alleles in an otherwise WT background. 4Heterozygous diploids expressing a single copy of the indicated REC114 or rec114 alleles; the other allele is rec114Δ.