Supplementary Material for: Migration of Polyphosphate Granules in <b><i>Agrobacterium tumefaciens</i></b>
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<i>Agrobacterium tumefaciens</i> has two polyphosphate (polyP) kinases, one of which (PPK1<sub>AT</sub>) is responsible for the formation of polyP granules, while the other (PPK2<sub>AT</sub>) is used for replenishing the NTP pools by using polyP as a phosphate donor to phosphorylate nucleoside diphosphates. Fusions of eYFP with PPK2<sub>AT</sub> or of the polyP granule-associated phosin PptA from <i>Ralstonia eutropha</i> always co-localized with polyP granules in <i>A. tumefaciens</i> and allowed the tracking of polyP granules in time-lapse microscopy experiments without the necessity to label the cells with the toxic dye DAPI. Fusions of PPK1<sub>AT</sub> with mCherry formed fluorescent signals often attached to, but not completely co-localizing with, polyP granules in wild-type cells. Time-lapse microscopy revealed that polyP granules in about one-third of a cell population migrated from the old pole to the new cell pole shortly before or during cell division. Many cells de novo formed a second (nonmigrating) polyP granule at the opposite cell pole before cell division was completed, resulting in two daughter cells each having a polyP granule at the old pole after septum formation. Migration of polyP granules was disordered in mitomycin C-treated or in PopZ-depleted cells, suggesting that polyP granules can associate with DNA or with other molecules that are segregated during the cell cycle.
