Peptides and fragments from digested BmrA in DDM detected using APPI-MS.

The native protein BmrA was purified in 0.05% of the non ionic detergent beta-D-dodecyl maltoside (DDM) in the presence of salts (50 mM Tris/HCl and 50 mM NaCl). In-solution trypsin digestion was performed and the mixture was analyzed by in infusion on APPI–MS without any additional sample preparation. Ions obtained from the peptides (P) or fragments (a-, b-, c- or y-) are reported with the position along the protein sequence.