Vitis vinifera Transcriptome or Gene expression. Vitis vinifera

Pinot Meunier L1 dwarf grapevines were grown in a growth cabinet and both unripe (preveraison) and ripe (postveraison) berries were collected after various time points post heat shock treatment. The gene expression profiles of treated berries were compared to controls to identify gene expression changes associated with the heat shock response. Keywords: Heat shock treatment effect Overall design: The effect of heat shock treatment on gene expression for preveraison and postveraison grape berries at seven time points was analyzed using the Affymetrix one colour array platform. Microarray data was obtained for berries on L1 mutant plants grown in a growth chamber at an ambient temperature of 16 degrees. They were heat shocked for 7 hours and then returned to ambient temperature. Berry samples were taken at 0, 1, 4, 7, 9, 14 and 24 hours after heatshock treatment. Two biological replicates were collected for each treatment. Biotin labeled probes were prepared from the extracted RNA from each deseeded berry sample and hybridized to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridization station was randomized using the AlgDesign package from R.