OF EVENTS to GLYPHOSATE TOLERANCE IN ALFALFA and METHODS FOR DETECTION

Of alfalfa events J-101 and J-163， and of DNA molecules unique for these events. The present invention also provides methods for detecting the presence of molecules of DNA in an plant sample. Claim 1: Seeds of plants of alfalfa， characterized in that its name is J-101 and its representative seeds are deposited in the Collection of Types of American Culture (II) with the Number of accession number PTA-4814. 5: The claim of alfalfa plant J-101 of the claim 4， characterized in that the genome of said plant of alfalfa J-101 comprising one molecule of DNA that are selected from the group which consists in the SEQ ID no: 1， SEQ ID no: 2， SEQ ID no: 3 and SEQ ID no: 4. Claim 10: One method for producing an plant that tolerates the application of glyphosate herbicide， characterized in that comprising: (to) by sexually crossing One first of one parent event of alfalfa with tolerance to glyphosate J-101， and one second of one parent of alfalfa plant that lacks the tolerance to glyphosate， of Thereby one of plurality of the progeny plants; and (b) selecting one of the progeny plant that tolerates the application of glyphosate; and (c) autocruzar the plant of the first Progeny， thereby one of plurality of the second progeny plants; and (d) select between those of the second progeny plants those plants that exhibit tolerance to glyphosate. Claim 11: One method for detecting the presence of DNA that corresponds to one of the DNA of alfalfa plant J-101 in one sample， characterized in that comprising: (to) put the Shows that DNA comprising in contact with one set of primers which， when they are used in one reaction of amplification of nucleic acids with the genomic dna of alfalfa event J-101， Produces one amplicon of which comprising the DNA SEQ ID no: 1 or SEQ ID no: 2; and (b) performing one of amplification reaction of nucleic acids， thereby producing the amplicon; and (c) detecting the amplicon. Claim 12: One method for detecting the presence of DNA that corresponds to one alfalfa J-101 in one sample， characterized in that comprising: (to) put the sample comprising DNA which is contact with an Probe， are that hybridizes under conditions of stringent hybridization with the genomic dna of alfalfa event J-101 and does not hybridize under conditions of hybridization are strict with DNA of one of alfalfa plant control， Where said probe is homologous or complementary with SEQ ID no: 1 or SEQ ID no: 2; and (b) subjecting the sample and probe to the conditions of stringent hybridization; and (c) detecting the hybridization of the probe with the DNA.