Analysis of cabazitaxel-resistant mechanism in human castration-resistant prostate cancer

To investigate cabazitaxel (CBZ) resistance in prostate cancer, we examined the changes in global gene expression before and after development of acquired CBZ resistance. Functional annotation clustering (FAC) analysis of DAVID showed cell division (GO: 0051301) and mitotic nuclear division (GO: 0007067) were the most enhanced clusters in DU145CR compared with DU145. DU145 and DU145CR cells were used for microarrays. DU145CR cells were newly established in our laboratory from DU145 cells as an acquired cabazitaxel resistant subline, which were grown and passaged upon reaching confluence in medium containing CBZ over a 18-month period.