SRRM4 knockout helps the human mesenchymal stem cell line to intrude into decellularized bone

Analysis of the human bone marrow microenvironment requires an in vivo model that reflects this microenvironment. We introduced a human bone marrow mesenchymal stem cell (MSC) line into decellularized bone. Using the CRISPR Knock Out library, we identified serine/arginine repetitive matrix 4 knockout in an MSC cell line, as a candidate factor for bone intruding, which may be applied to the analysis of hematological diseases such as myelodysplastic neoplasm. RNA-seq for Control UE7T-9 cell line(human mesenchymal stem cell line) and SRRM4 KO UE7T-9cell line. SRRM4 KO was induced using CRISPR.