Microarray expression data of AtCRK1 (At2g41140) knockout line, overexpression line 34 and overexpression line GFP on zero mM NaCl and 100 mM NaCl stress levels. CEL files are the original microarray files. Text files contain raw and analyzed data of the knockout line and overexpression lines.
收藏DataCite Commons2025-10-22 更新2026-04-25 收录
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https://figshare.com/articles/dataset/Microarray_expression_data_of_AtCRK1_At2g41140_knockout_line_overexpression_line_34_and_overexpression_line_GFP_on_zero_mM_NaCl_and_100_mM_NaCl_stress_levels_CEL_files_are_the_original_microarray_files_Text_files_contain_raw_and_analyzed_da/30414628/1
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For whole genome expression analysis, we used Affymetrix [GeneChip<sup>®</sup>] (Thermo Fisher Scientific, Waltham, MA, USA) microarray analysis. For this purpose, total RNA was extracted from callus and leaves of wildtype, mutants, AtCRK1-overexpression (OX) line, and AtCRK1 KO line using a RNeasy Plant Mini Kit (Qiagen). RNA was treated with RT-grade DNase and quantified through a bioanalyzer (Agilent 2100). cDNA was prepared using 2 μg of RNA following the standard GeneChip<sup>®</sup> protocol for eukaryotic target preparation (Affymetric GeneChip<sup>®</sup> Expression Analysis Technical Manual) (http://tools.thermofisher.com/content/sfs/manuals/expression_analysis_manual.pdf). All cDNA cleaning, cRNA preparation, cRNA quantification, cRNA fragmentation, hybridization, staining, washing, and scanning were performed using standard GeneChip<sup>®</sup> expression protocols. Microarray data were analyzed with Partek Genomics Suite<sup>®</sup> software.
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figshare
创建时间:
2025-10-22



