Expression data from MDSC treated with STAT3 inhibitor Napabucasin. Expression data from MDSC treated with STAT3 inhibitor Napabucasin

MDSC (myeloid-derived suppressor cells) were generated in vitro using IL-6 and GM-CSF. To identify the role of STAT3 in MDSC, we treated the generated cells for 24 hours with DMSO (control) or 1 µM STAT3 inhibitor Napabucasin (also known as BBI608) and compared their expression profile by microarray. We found genes and pathways that are up- or downregulated upon STAT3 inhibition in MDSC. Overall design: Total RNA was isolated from in vitro generated MDSC after 24 hours of DMSO (control) or 1 µM Napabucasin treatment and hybridized to Affymetrix microarrays. To generate biological replicates, MDSC generated from the bone marrow of three different mice were used.