Renal circadian rhythm dysregulation in lupus nephritis [RNA-seq]

This study examines the circadian gene expression of whole kidneys of young and nephritic NZB/W F1 mice Overall design: Young mice were harvested at 12 weeks of age. Nephritic mice were harvested 2-3 weeks after nephritis onset (>300mg/dl proteinuria) but before the development of endstage renal failure (BUN >25). Remission was induced in nephritic mice with new onset proteinuria of less than 1 week duration measured twice 24 hrs apart using one dose of cyclophosphamide 50mg/kg and 6 doses of CTLA4Ig (200ug) and anti-CD40L (250ug) given over 2 weeks. Kidneys were harvested 8 weeks later from mice still in remission with proteinuria 30mg/dl. Kidneys were perfused with PBS and total RNA was isolated from perfused mouse kidneys using TRIzol reagent