SCFAs data.xlsx

Fecal samples from mice fed a normal diet (DW) or DKT diet (DW+DKT) for 21 days (on day 0) were subjected to GC/MS. Metabolites in the cecal contents were analyzed by GC/MS-TQ8040 (Shimazu, Kyoto, Japan) with a BPX-5 column (30 m × 0.25 mm i.d.; film thickness 1.00 mm, Trajan Scientific and Medical, Vic., Australia) for SCFAs, as described previously (53). Mass spectrum peaks were detected using the GC/MS solution software (Shimazu), and the retention time correction of peaks was performed based on the retention time of a standard alkane series mixture (C9 to C33). Metabolites were identified by the Smart Metabolites Database (Shimazu), which contains multiple reaction monitoring transitions for 12 metabolites commonly found in biological samples for BPX-5.<br>