20240305 Gray Figure 6 data

<b>FIG 6 Glutathione reductase contributes to </b><b><i>E. coli </i></b><b>HOSCN resistance. </b><i>E. coli</i> strains MG1655 (wild-type), MJG0044 (MG1655 ∆<i>nemA728</i>), MJG0081 (MG1655 <i>hslO</i>::<i>kan</i>⁺), MJG0176 (MG1655 ∆<i>mgsA778</i>), MJG1958 (MG1655 ∆<i>rclA747</i>), MJG2371 (MG1655 ∆<i>trxB786</i>::<i>kan</i>⁺), MJG2385 (MG1655 ∆<i>trxA732</i>::<i>kan</i>⁺), MJG2387 (MG1655 ∆<i>trxC750</i>::<i>kan</i>⁺), MJG2388 (MG1655 ∆<i>grxA750</i>::<i>kan</i>⁺), MJG2398 (MG1655 ∆<i>gor-756</i>::<i>kan</i>⁺) were grown in MOPS minimal medium without micronutrients containing 0.2% glucose, with or without 400 µM HOSCN, as indicated, incubating at 37°C with shaking and measuring A₆₀₀ at 30-minute intervals for 24 hours (n=3 experimental replicates, with 3-4 technical replicates per experiment; error bars of 1 standard deviation).