Large-scale purification of HTT domain constructs 2019/04/01

<strong>Project: </strong>High resolution structural analysis of purified HTT samples <strong>Experiment: </strong>Large-scale purification of HTT domain constructs <strong>Date completed:­ </strong>2019/04/01 <strong>Rationale: </strong>Domain fragments of the huntingtin protein are useful reagents for examining protein-protein interaction characteristics of huntingtin and to map interaction interfaces. Stable, monodisperse and pure samples may also be amenable to high resolution structure solution by X-ray crystallography. Previously huntingtin fragments were cloned and screened for expression in small-scale (3 mL) culture experiments. Positive hits were scaled for production as detailed in the table on the next page. All previous work is described in this post:https://zenodo.org/record/2600051#.XKU89aeZPOQ.