five

Rapid tRNA decay can result from lack of non-essential modifications

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NIAID Data Ecosystem2026-03-07 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE3503
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A microarray tiling approach was utilized to assay tRNA modifications by comparing hybridization affinity of tRNA-modification mutants to wild-type across all annotated yeast tRNAs. Keywords: Assaying noncoding RNA modifications Complementary probes were tiled on average every 5 bases across all tRNAs as well as other noncoding RNAs including 50 bases of flanking genomic sequence. Probes were normalized to a uniform melting temp of 55 degrees celsius and concatenated to 60 bases.
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2012-03-16
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