RNA-seq analysis for Chlamydomonas in H82 (cas mutant)

To identify genes affected by the CAS mutation, RNA-seq analysis was performed in C9 (WT), H82 (CAS mutant), and W48 (C-1) strains. RNA was extracted from cells cultured under high-CO2 (HC) or Very low-CO2 (VLC) for 0.3 h or 2 h. There are two biological replicates of each condition. Sequencing was performed on HiSeq 2500 system, and consisted of forward and reverse reads.