Cardiac profiling of Yap-bound chromatin in neonatal Salvador mutant mice

In this study, we identified chromatin regions bound by YAP, a major effector of the Hippo tumor suppressor pathway. To disrupt Hippo signaling in the mouse heart, we inactivated the single mammalian Salv ortholog using a Salv conditional null allele and and tamoxifen inducible Myh6-cre/Esr1 allele, Cre activity was induced with 4 consecutive intraperitoneal (i.p.) or subcutaneous injections of tamoxifen from P7-P10. Genomic DNA from P8-stage and P12-stage hearts were used for Yap ChIP-seq.