CD74-NRG1 fusion as an bona fide oncogene

3' RNA sequencing results of Rosa26CD74-NRG1/Flp mice (murine embryonic fibroblasts, tumor and normal tissue) Overall design: To test this newly generated mouse model, we crossed Rosa26FSF.CD74-NRG1 mice with an established FLP-deleter strain (Rosa26Flp), which results in early embryonic excision of the FRT-flanked stop cassette and in nearly subsequent ubiquitous expression of the CD74-NRG1 fusion protein in all tissues of the resulting Rosa26FSF.CD74-NRG1/Flp mice [20]. To generate murine embryonic fibroblasts (MEF) out of these Rosa26FSF.CD74-NRG1/Flp mice, Rosa26FSF.CD74-NRG1/Flp mice carrying the CD74-NRG1 transgene as well as the FLP deleter allele were mated. We then used embryos homozygous (Rosa26FSF.CD74-NRG1/FSF.CD74-NRG1) and heterozygous (Rosa26FSF.CD74-NRG1/Flp) for the transgene, as well as CD74-NRG1 negative embryos (Rosa26Flp/Flp) for MEF generation. To characterize the different tumors further by transcriptome sequencing we compared tumor tissue of Rosa26CD74-NRG1/Flp mice to different normal tissue samples from individual animals.