Next Generation Sequencing facilitates to identify the differential expression genes of expanded MAIT cells upon the stimulation of 5-OP-RU/MR1 tetramer with/without GCDCA/TCDCA/UDCA

We enriched expanded MAIT cells in PBMCs from healthy donor upon the stimulation of 5-OP-RU/MR1 tetramer with/without GCDCA/TCDCA/UDCA through flow sorting to perform next-generation sequencing (NGS). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with illumina nova seq and sequence analysis was determined using the Illumina data analysis pipeline. Differential gene expression between expanded MAIT cells,expanded MAIT cells with GCDCA,expanded MAIT cells with TCDCA,expanded MAIT cells with UDCA.