Bulk RNA-Seq analysis of CD8+ T cells from treatment-naïve matched tumor-bearing lungs and spleens from the HKP1 orthotopic mouse lung adenocarcinoma model

To investigate how gene expression profiles change in CD8+ T cells based on time, tumor burden, and tissue localization, we orthotopically implanted the mouse lung adenocarcinoma HKP1 cell line expressing luciferase into syngeneic C57BL/6 mice by tail vein injections. Tumor growth was monitored by bioluminescence imaging. A separate cohort of mice received no tumor implantation. At days 7, 17, and 24 post tumor implantation, mice were euthanized and CD8+ T cells sorted from tumor-bearing lungs and spleens, or matched naive lungs and spleens. We then performed gene expression profiling analysis using data obtained from RNA-seq of sorted cells, comparing across time, tumor burden, and tissue localization. Comparative gene expression profiling of CD8+ T cells isolated from naïve lungs, naïve spleens, tumor-bearing lungs, or spleens of tumor-bearing mice, grouped by time post-tumor implantation (naïve [n=3], day 7 [n=6], day 17 [n=6], and day 24 [n=5]) and tumor burden (naïve [n=3], day 7 [n=6], low [n=6], high [n=5]).