RNAseq of PURA knockdown in HeLa cells

Here, we show that PURA predominantly resides in the cytoplasm, where it binds to a large set of transcripts. Many of these transcripts change abundance in response to PURA depletion, in parts reflected in altered protein expression levels. A closer inspection of the regulated proteins indicates a role of PURA in immune responses, mitochondrial function, autophagy and processing (P)-body activity. Overall design: Poly A+ RNAseq of HeLa cells with 4 PURA siRNA knockdown and 4 control samples.