TCNAs-mediated caspase activation.

HL-60 cells were incubated with DOXY or MINO in final concentrations of 2.5, 10 and 25 µg/ml or COL-3 in final concentrations of 0.75, 1 and 5 µg/ml for 2, 4, 6 and 24 h. Cells incubated in DMSO in a final concentration of 0.2% were used as controls for solvent toxicity and cells incubated in complete medium served as controls. Cells incubated with etoposide in a final concentration of 6 µg/ml served as positive controls for apoptosis. The protein band densities were estimated using the Image J software and each band density in proportion to the control was calculated. The presence of cleaved bands was used as an indicator of caspase activation. The fold changes in proportion to the controls were arranged in the following score to indicate the degree of activation: 5–15 (+), 16–30 (++) 31–60 (+++) and over 60 (++++). ND: not done.TCNAs-mediated caspase activation.