Additional file 2 of Systematic discovery of subcellular RNA patterns in the gut epithelium
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Additional files 2: Supplementary Tables 1-27. Table S1. Differential expression analysis results between DPP4 and GFP tethered APEX sequencing. Table S2. Categorized GO terms and Counts for the each categorized GO term. Table S3. GSEA of the APEX-seq data from DE results of DPP4. Table S4. Normalized gene counts of Mitochondria tagged samples. Table S5. Differential gene expression analysis results between ACTB and GFP and GO analysis results for ACTB enriched genes compared to GFP in APEX2 seq data. Table S6. Comparison between Apex2-seq and LCM data from [2]. Table S7. Z-score values of the selected transcripts from APEX sequencing. Table S8. smFISH quantification data using spot detection analysis. Table S9. smFISH quantification data of each ROI from the gradient spot detection analysis. Table S10. Quantification of diameter of RNA granules. Table S11. RBP-MS data comparison between Lct-3'UTR and Fluc. Table S12. RBP-MS data comparison between Net1-3'UTR and Fluc. Table S13. MERFISH 500 gene panel with barcode IDs. Table S14. Volume normalized MERFISH dot count differences between apical and basal sides of sIOs sections. Table S15. Shared gene list from UpSet plot on APEX-seq and sIOs-MERFISH data. Table S16. Wilcoxon test on sIOs-MERFISH data between apical and basal regions per gene. Table S17. Volume normalized MERFISH dot count differences between apical and basal sides of sIT sections. Table S18. Volume normalized MERFISH dot count for whole cell mass for sIT. Table S19. Venn diagram of sIOs and sIT (apical, basal and intersections). Table S20. Volume normalized MERFISH dot count differences between apical and basal sides of lIT sections. Table S21. Volume normalized MERFISH dot count for whole cell mass for lIT. Table S22. The scaled RNA-Protein plot with the GO categorization for villus Top and villus Bottom. Table S23. Plasmid IDs and sequences for APEX-seq and 3'UTR work. Table S24. qPCR results and primer information for mito APEX-seq. Table S25. Primer sequences for in vitro transcription. Table S26. Plasmid IDs and sequences for shRNA work. Table S27. qPCR results and primer information for shRNA work.
创建时间:
2025-10-29



