RNA-seq of Westar seedlings treated with Spd or freezing stress

To further reveal the underlying regulatory mechanism of Spd in the freezing response of rapeseed seedlings, Westar seedlings were treated with ddH2O (NSpd_C) and 1.0 mM Spd (HSpd_C) under normal conditions, and with ddH2O (NSpd_F) and 1.0 mM Spd (HSpd_F) under freezing stress conditions. After RNA extraction from the tissues, cDNA libraries were constructed and subjected to transcriptome sequencing.