nsp15 binds nsp8

This COVID-19 event has been created by a combination of computational inference (see https://reactome.org/documentation/inferred-events) from SARS-CoV-1 data and manual curation, as described in the summation for the overall SARS-CoV-2 infection pathway.<br><br>Nonstructural protein 15 (nsp15) of the SARS coronavirus (SARS-CoV-1) binds to the replication-transcription complex (RTC) through interaction with nsp8 (Imbert et al. 2008). This interaction appears to be conserved in other coronaviruses, such as mouse hepatitis virus (MHV) (Athmer et al. 2017). nsp15 is an endonuclease characteristic for the order Nidovirales that includes the family Coronaviridae. nsp15 preferentially cleaves 3' of uridines, generating 2'-3' cyclic phosphates after cleavage. nsp15 requires Mn2+ ions for catalytic activity. Functional nsp15 is needed for production of viable virions and for viral transcription (Guarino et al. 2005, Ricagno et al. 2006, Bhardwaj et al. 2006, Joseph et al. 2007, Bhardwaj et al. 2008, Bhardwaj et al. 2012). The biological role of nsp15 has not been elucidated. It may degrade host mRNAs to shut down host translation, but so far no human or viral RNA targets have been identified.

本COVID-19事件由SARS-CoV-1数据经计算推理（参见https://reactome.org/documentation/inferred-events）及人工编纂相结合而创建，具体编纂方法详见SARS-CoV-2感染途径的整体概述。<br><br>SARS冠状病毒（SARS-CoV-1）的非结构蛋白15（nsp15）通过与其nsp8（Imbert等，2008年）相互作用而与复制转录复合体（RTC）结合。这一相互作用在其他冠状病毒，如小鼠肝炎病毒（MHV）（Athmer等，2017年）中似乎得到了保留。nsp15是Nidovirales目（包括冠状病毒科）中特有的内切酶。nsp15优先切割尿苷的3'端，切割后在3'端生成2'-3'环状磷酸盐。nsp15需要Mn2+离子以发挥催化活性。功能性的nsp15对于生产有活力的病毒颗粒和病毒转录至关重要（Guarino等，2005年，Ricagno等，2006年，Bhardwaj等，2006年，Joseph等，2007年，Bhardwaj等，2008年，Bhardwaj等，2012年）。nsp15的生物学作用尚未阐明。它可能通过降解宿主mRNA来终止宿主翻译，但迄今为止，尚未识别出人类或病毒的RNA靶标。