Study of lipase production by Burkholderia cepacia

ABSTRACT The use of enzymes on an industrial scale has enabled the development of technological processes with an efficiency similar to those made by nature, which makes this technology one of the most promising fields in new technologies for synthesis of high-added value compounds. This paper aimed to study the production of lipase by Burkholderia cepacia using the response surface methodology. We used the following variables: concentrations of potassium, magnesium, soybean oil, corn steep liquor and pH. We observed that, among the used concentrations, potassium, corn steep liquor and soybean oil positively influenced the lipase production. Bioflo III showed the best performance among the bioreactors used for the enzyme production, possibly due to a better distribution of mass and movement transfer phenomena, reaching values of up to 2.43 U mL-1 at 120 hours of fermentation.