RNA-sequencing results of MDA-MB-231 with/without OGFOD1

Recently, high levels of OGFOD1 has been reported in variety of cancers. Despite of the significances, the precise mechanism is poor understood. In order to find how OGFOD1 affects the cancer development, we conducted RNA-sequencing in MDA-MB-231. OGFOD1 was knocked out using CRISPR/Cas9 system. Total mRNA was isolated from parental and OGFOD1-knockout (OGFOD1?/?) MDA-MB-231 cells. Isolated RNA was used to prepare an mRNA sequencing library using TruSeq Stranded mRNA sample preparation kit. All the samples were sequenced on Illumina NextSeq 500 Sequencer with a 75 bp paired-end High Output kit. Overall design: mRNA profiles of parantel and OGFOD1 KO MDA-MB-231 cells.