Expression data of liver resident cells from C57BL/6 mouse

The liver is composed of various resident cells, including hepatocytes, Kupffer cells, liver sinusoidal endothelial cells (LSEC), and hepatic stellate cells (HSC). Hepatocytes are the primary type of liver cells, constituting 80% of the liver's mass, and are responsible for tasks such as protein and lipid synthesis, detoxification, and bile secretion. Kupffer cells, on the other hand, are liver-resident macrophages that play a crucial role in detecting foreign antigens and danger signals within the liver, as well as clearing apoptotic cells. LSEC is in charge of blood vessel formation in the liver and is responsible for eliminating cell debris. In the quiescent state, HSC stores vitamin A and is essential in liver fibrosis when activated. Freshly isolated HSCs undergo automatic activation upon culture. In this study, we present microarray data for freshly isolated mouse hepatocytes, Kupffer cells and LSEC, along with HSCs that have been cultured for 1, 7 or 14 days. Freshly sorted hepatocytes, Kupffer cells, LSECs and culture-activated HSCs were used to obtain microarray data. To culture-activate HSC, the cells were maintained in DMEM supplemented with 10% FBS in a 37°C, 5% CO2 humid incubator for 1, 7, or 14 days. All cells were isolated from naïve C57BL/6 mice. For hepatocytes, Kupffer cells, and LSEC, three biological replicates were included, while one-day cultured HSC had two replicates.