Single-cell transcriptomic analysis of zebrafish fli1b mutants

To characterize fli1b role in zebrafish hematopoietic development, we obtained samples from zebrafish fli1b mutant and control wild-type embryos at 23-24 hpf stage. Whole embryo single-cell RNA-seq demonstrated reduction in the expression of hematopoietic markers associated with erythroid and myeloid development. A subset of vascular endothelial markers was also reduced in fli1b mutants. These results demonstrate fli1b role in hematopoietic and vascular development. Approximately 40 homozygous fli1b-/- and wild-type zebrafish embryos at 23-24 hpf stage were obtained from incrosses of fli1b-/- or sibling wild-type parents, respectively. All cells were disaggregated and subjected to single-cell RNA-seq using 10x Genomics Chromium system.