Supplementary tables and processed datasets

To explore the cellular heterogeneity in the developing and functionally mature thymus, we constructed an integrative transcriptomic atlas of human thymocytes involving both prenatal and postnatal stages. Specifically, we characterized the following aspects: (i) defining the prenatal DN subsets transcriptomically, (ii) revealing the transcriptional differences between prenatal and postnatal DN cells regarding key genes expression, enriched GO terms as well as metabolic programs, and (iii) illustrating the different preference of polyadenylation sites usage in prenatal and postnatal thymocytes at single-cell resolution. Supplementary tables and datasets include the re-processed datasets, integrated annotation of each cell cluster and subcluster, UMAP dimensionality reduction, scaled regulon activities, pesudotime inferred by Monocle 3, and patterns genes.