Investigating the role of Foxp1 in murine A20 lymphoma cells

Purpose: to investigate the role of transcription factor Foxp1 in murine A20 lymphoma cells, which can be used as a model for human diffuse large B cell lymphoma Method: Exons 6 and 7 of Foxp1 were independently targeted using CRISPR/Cas9 editing, and 2 clones from each targeted exon (E6 and E7), showing depleted Foxp1 isoforms were isolated. Method: RNA sequencing was conducted on parental A20 cells and CRISPR clones, and differential gene expression was undertaken The mRNA fraction of total RNA from A20 parental cells and two independent CRISPR clones was sequenced in triplicate using Illumina HiSeq4000