Transcriptome profiling of CML24-mediated Al resistance

To understand the molecular network underlying the regulation of ALMT1 under Al stress by CML24, the transcriptional profile of wild type and cml24-2 mutant roots in the absence or presence of Al stress was examined using RNAseq.In roots of wild type, 1173 and 1493 genes were up- or down-regulated, respectively, by Al, while 919 and 1057 genes were up- or down-regulated, respectively, in roots of cml24-2 plants. Totally 382 and 176 genes were up- or down-regulated, respectively, at least 2-fold in the roots of the cml24-2 mutant compared with wild type plants in absence of Al. In presence of Al, a total of 412 genes were up-regulated and 178 genes were down-regulated at least 2-fold comparing the cml24-2 mutant with wild type plants. Total RNA obtained from control (non-treated roots) and Al-exposed roots. Six day old plants were treated with and without Al treatment for 6 h, then total RNA was isolated using the RNeasy® Plant Mini Kit (QIAGEN). RNAseq analysis was performed by BGI Tech (Shenzhen, China) via BGISEQ-500 RNA-Seq platform.