Interaction Proteomics Reveals Brain Region-Specific AMPA Receptor Complexes

Fast
excitatory synaptic transmission in the brain is mediated by glutamate
acting on postsynaptic AMPA receptors. Recent studies have revealed
a substantial number of AMPA receptor auxiliary proteins, which potentially
contribute to the regulation of AMPA receptor trafficking, subcellular
receptor localization, and receptor gating properties. Here we examined
the AMPA receptor interactomes from cortex, hippocampus, and cerebellum
by comprehensive interaction proteomics. The study reveals that AMPA
receptor auxiliary proteins are engaged in distinct brain region-specific
AMPA receptors subcomplexes, which might underlie brain region-specific
differential regulation of AMPA receptor properties. Depending on
the brain region, an interacting protein can be involved in an AMPA
and a non-AMPA receptor complex.