Two novel species of Hermatomyces (Dothideomycetes) from Fagales in China

The phylogenetic analyses of the combined LSU, ITS, TEF1α, and RPB2 dataset comprised 64 taxa. The dataset consisted of 3325 characters, including gaps (LSU: 1-851 bp, ITS: 852-1379 bp, TEF1α: 1380-2313 bp, RPB2: 2314-3325 bp). The matrix had 1239 distinct alignment patterns, with 20.68% of undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.245345, C = 0.263963, G = 0.263735, T = 0.226957; substitution rates: AC = 1.185929, AG = 4.922882, AT = 1.461014, CG = 0.926895, CT = 11.037710, GT = 1.000000; gamma distribution shape parameter α = 0.673356. The best-scoring RAxML tree with a final likelihood value of -18165.231993 is presented in Fig. 1.In the multi-locus phylogenetic tree (Fig. 1), our isolates were distributed through the tree, representing two new species and one newly collected species. Hermatomyces yunnanensis (HKAS 144361 and HKAS 144362) formed a basal clade to H. constrictus Koukol & G. Delgado (CCF 5904), H. griseomarginatus Koukol & G. Delgado (PRC 7203 and PRC 7201), H. verrucosus Koukol & G. Delgado (CCF 5903 and CCF 5892) and H. sphaericoides Koukol & G. Delgado (CCF 5908 and CCF 5895) with maximum statistical support (MLBS = 100%, BPP = 1.00). Hermatomyces nujiangensis (HKAS 144367 and HKAS 144368) formed a sister clade to H. pyriformis H.Z. Du & Jian K. Liu (HKAS 132457 and HUEST 23.0442) with strong support (MLBS = 93%, BPP = 1.00). Hermatomyces hainanensis Jian Ma, Y.Z. Zhang bis & Y.Z. Lu (HKAS 144351 and HKAS 144352) grouped with H. hainanensis (GZCC 23-0592) with strong support (MLBS = 92%, BPP = 1.00).