DNA hydroxymethylation profiling of purple sea urchin (Strongylocentrotus purpuratus), european lancelet (Branchiostoma lanceolatum) and zebrafish (Danio rerio) using hMeDIP-seq

Using hMeDIP-seq we validated the single-base resolution hydroxymethylomes (ACE-seq) of sea urchin, lancelet and zebrafish embryos. hMeDIP-seq was perfomred on (i) purple sea urchin 72 hpf embryos; (ii) european lancelet 36 hpf embryo; (iii) zebrafish 24 hpf embryo (in replicates). Input DNA sequencing has been performed in parallel.