秸秆类生物质绿色拆解及C5-C6 糖平台构建实验数据集
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四川农业大学成都校区建立秸秆类生物质绿色拆解核心技术示范区,应用蒸汽爆破与低共熔溶剂两种预处理技术。针对秸秆拆解难题,按TAPPIT222om-02标准及研究测试方案,开展两种预处理实验对比,测试预处理后秸秆底物性质及结构变化,综合分析综纤维素回收及木质素结构保留等指标。C5-C6 糖平台构建研究数据来自中科院广州能源研究所相关研究室搭建的实验平台及分析测试中心,按照研究内容和研究测试方案,采集不同预处理甘蔗渣综纤维素宏观、微观特征及纤维素酶系,酶复配综合分析综纤维素酶解糖化效率指标。中国科学院广州能源研究所生物质生化转化研究室搭建平台,数据内容包括探究不同预处理方法对秸秆拆解效果,获高综纤维素且适配酶解的预处理物料相关数据;开发预处理综纤维素底物适配的复合酶,获综纤维素酶解糖化效率指标,以最优复合酶添加的高固反应体系构建C5-C6糖平台数据等。
A core technical demonstration zone for green disassembly of straw-based biomass was established at the Chengdu Campus of Sichuan Agricultural University, where two pretreatment technologies, steam explosion and deep eutectic solvents, were applied. Aiming at the challenge of straw disassembly, comparative experiments of the two pretreatment methods were conducted in accordance with the TAPPIT222om-02 standard and research testing protocols. The properties and structural changes of pretreated straw substrates were tested, and indicators including holocellulose recovery and lignin structure retention were comprehensively analyzed. The research data for C5-C6 sugar platform construction comes from the experimental platform and analytical testing center built by the relevant research laboratory of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences (CAS). According to the research content and testing protocols, the macroscopic and microscopic characteristics of holocellulose from pretreated bagasse, as well as cellulase systems, were collected, and the enzymatic saccharification efficiency of holocellulose was comprehensively analyzed via enzyme compounding. The platform was built by the Laboratory of Biomass Biochemical Conversion, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences. The data content includes: relevant data on exploring the effects of different pretreatment methods on straw disassembly to obtain pretreated materials with high holocellulose content and suitability for enzymatic hydrolysis; data on developing composite enzymes adapted to pretreated holocellulose substrates and obtaining the enzymatic saccharification efficiency of holocellulose; data on constructing the C5-C6 sugar platform using high-solid reaction systems with optimal composite enzyme addition, etc.




