Vector switch to eliminate false positive clones.
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+LgT and Lam+LgT were used as positive and negative controls, respectively. Necdin was switched from the prey vector (pGADT7) to the bait vector (pGBKT7). The new constructs were re-tested and their interactions were validated. 3-AT: 3-amino-1,2,4-triazole, a competitive inhibitor of the His3 protein. p53
创建时间：
2015-12-02
