Sequencing of serum exosomal circRNAs in colorectal cancer

CircRNA sequencing for 10 samples To assess the serum exosomal circular RNAs (circRNAs) expression profile and explore the potential functions in human colorectal cancer (CRC).The differentially expressed circRNAs profiles of CRC (n=5) and age-matched healthy controls(n=5) were analyzed using RNA-seq profiling. Quantitative real-time PCR was used to validate the expression pattern of circRNAs.Bioinformatic tools including network analysis, Gene ontology, and KEGG pathway analysis were utilized. Overall design: Sequence was performed on 5 colorectal cancer samples and 5 healthy control samples,each sequencing sample was a mixture of 10 serum from participant.