Microbiota of intestinal contents of dissected mice

Whole intestines of 6 mice were dissected into 6 sections (SI1, SI2, SI3, SI4, Cecum, LI). The intestinal tract contents were washed out using 1ml of PBS buffer and subjected to enzyme-based DNA extraction process. The 16S rRNA gene hypervariable V1-V2 regions were PCR-amplified using universal primers (a barcoded 27Fmod and 338R) and, multiplexed amplicon pyrosequencing was carried out using 454 GS FLX Titanium or 454 GS JUNIOR (Roche Applied Science).