RNA-seq of Bacillus subtilis WT W168 treated with the antimicrobial peptide YydF (0.5µM) compared to untreated control

To obtain global cellular response of Bacillus subtilis WT W168 against the intrinsically produced antimicrobial peptide YydF, we performed RNA-seq experiments. For this, we synthesized YydF, extrinsically added 0.5µM to logarithmic phase growing cells and harvested cells after 10 min exposure. Experiments were performed in triplicates and non-induced Bacillus subtilis WT W168 was used as reference condition. Overall design: Examination of YydF induced Bacillus subtilis WT cells compared to non-induced Bacillus subtilis WT cells