Phylogenetic identification and distribution of bacteria excised and sequenced from DGGE bands.
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Identification of the dominant bands in the PCR-DGGE fingerprinting profiles (marked in Fig. S1) and their distribution in the different fractions of the pepper root system. The codes of the different fractions of the pepper root systems are as follow: E, Endosphere; R, rhizosphere; S, root-surrounding soil; B, non-cultivated root-free arid soil. The numbers following the codes indicate the different replicates.
X: presence of the band in the DGGE profile of the indicated fraction; in bold are indicated the bands that were actually sequenced.
Sequences of bands with the same mobility in the DGGE gel are reported in the same white/grey boxes. In some cases the different bands showed slightly different sequences whit few nucleotide variations. When the variation resulted within the 3% divergence on the 16S rRNA sequence, the bands where assumed to belong to the same OTU at the 97% identity threshold, as evaluated using DOTUR [68].
创建时间:
2012-10-31



