Multicolor labeling scheme.

aCPITC-conjugated phalloidin (Sigma-Aldrich Co.); bmouse IgM anti-α-actinin primary antibody (clone 75.2, catalogue number A5044, Sigma-Aldrich Co.); cisotype-specific Cy3-conjugated goat anti mouse-IgM secondary antibody; dmouse IgG anti-phosphotyrosine antibody (PT66, Sigma-Aldrich Co.); eAlexa-555-conjugated Fab fragments (Zenon kit, Molecular Probes Inc.); fmouse IgG anti-paxillin antibody (Transduction Laboratories, Lexington, KY, USA); gAlexa-750-conjugated Fab fragments (Zenon kit, Molecular Probes Inc., Eugene, OR, USA); hrabbit anti-vinculin antibody (clone R695 [34]); irabbit-anti-zyxin antibody (B71, kindly provided by Laura Hoffman and Mary Beckerle, Huntsman Cancer Institute and Department of Biology, University of Utah, Salt Lake City, UT, USA); jrabbit anti-FAK antibody (AHO0502, Biosource International Inc., CA, USA).