3-prim-UTR-Seq of chicken adipose tissue

3-prim UTR sequencing (3-prim-UTR-seq) is a powerful and simple method to measure mRNA quantitatively through sequencing a small fragment (e.g., 100 bases) at the 3-prim end of polyadenylated RNAs. This method has been proposed as a lower-cost alternative to RNA-seq to profile gene expression for differential expression (DE) analysis, as the number of reads generated by 3-prim-UTR-seq is expected to be proportional to the sample transcripts. Additionally, 3-prim-UTR-seq can bypass the biased estimation of expression levels in RNA-seq resulting from over-representation of long transcripts and save more sequencing space to increase the degree of multiplexing. This project utilized 3-prim-UTR sequencing to study gene expression in adipose tissue of chickens. The present study characterized and compared gene expression profiles in abdominal fat from broiler chickens of different feed efficiency (FE) levels to enhance the understanding of FE biology.