Exploring the inhibitory mechanism ofgastric cancer by Banxia Xiexin Decoctionthrough the combination of serumpharmacochemistry, networkpharmacology, and transcriptomics

The objective of this research is to identify differentially expressed genes (DEGs) in AGS and SUN cell samples treated with BXD-0 (0 mg/mL), BXD-L (0.5 mg/mL), and BXD-M (1 mg/mL) (n=3 per group). DEGs were analyzed both between treatment groups (e.g., BXD-L vs. BXD-0) and between cell lines (AGS vs. SUN). Overall design: RNA sequencing was performed on AGS and SUN cell samples divided into three groups: BXD-0 (0 mg/mL), BXD-L (0.5 mg/mL), and BXD-M (1 mg/mL) to identify differentially expressed genes (DEGs) and related pathways.