ChIP-seq of adenovirus core protein VII

To determine whether adenovirus core protein VII preferentially binds specific host chromatin regions, we performed native chromatin immunoprecipitation of protein VII-bound chromatin followed by next-generation sequencing (ChIP-seq). Native ChIP-seq was performed from chromatin purified from the diploid human cell line IMR-90 infected with WT adenovirus type 5 at MOI = 10 at 20 hours post infection. Native chromatin purified from Saccharomyces cerevisiae (as in PMID: 23468649) 4 hours post-galactose induction to express adenovirus protein VII was spiked in at 5% of the reaction. Chromatin IPs were performed for total histone H3 and protein VII using an endogenous antibody against the viral protein (PMID: 23468649). 10% input control was sequenced as well as H3 and VII ChIP libraries. Sequences were aligned to the human genome (hg38), S. cerevisiae (sacCer3), and human adenovirus type 5 (NCBI Reference Sequence AC_000008.1) to assess protein VII localization.